Our Methods
The McDonnell Lab focusses on an interdisciplinary approach to discovery science, as such we utilise a range of methods and collaborations to ensure use of cutting edge, novel methods. Below are our methods and links to the collaborators which make it possible.
Mass Spectrometry
Our MS work is facilitated by the Thalassinos Group at UCL.
Purification
Protein purification using AKTAs, Kingfisher instruments and OT-2 liquid handlers.
Western Blotting
We utilise the Bolt system and Biorad transfer processes, with visualisation on an Amersham ImageQuant
Molecular Simulation
We also specialise in simulation of protein structure and movement in artifical environments
Platform Proteomics
The McDonnell Lab also utilises a platform proteomic approach to diagnostics discovery at UCL.
Flow Cytometry
We also collaborate with cellular scientists and support Flow Cytometry methodologies through the UCL Rayne FACS Facility
ELISA
Alongside our structural work we also develop in-house and commercial ELISAs for large scale biomarker validation
Protein Production and Characterisation
We produce protein both in bacterial and mammalian cells, characterising it using biophysical approaches.